Haemoglobin

Persistent fetal haemoglobin and falsely high glycosylated haemoglobin levels.

Some of the routine methods of measuring glycosylated haemoglobin depend on its difference in charge from haemoglobin A and do not distinguish between glycosylated haemoglobin and fetal haemoglobin. Two insulin dependent diabetics showed persistent discrepancies between their capillary blood glucose values and their glycosylated haemoglobin values measured by agar gel electrophoresis: the blood...

Observations on foetal haemoglobin.

Foetal haemoglobin exhibits a much greater resistance towards alkaline denaturation than does adult haemoglobin, thus constituting an important distinction between the two types of haemoglobin. The phenomenon was first described by Korber (1866), observed again by Kruger (1887-88), and subsequently studied in more detail by Bischoff (1926), Haurowitz (1929, 1930), and Brinkman, Wildschut and Wi...

Intra-erythrocytic haemoglobin crystals.

When investigating a newly discovered haemoglobin L, using Itano's solubility test (Itano, 1953), a resemblance to sickle cell haemoglobin was noticed inasmuch as solubility was not complete in 2.24 M phosphate buffer. This was later realized to be due to an artefact (see Ager and Lehmann, 1957), but at that time the cells containing haemoglobin L were tested for sickling by incubation with red...

Measurements for haemoglobin.

Haemoglobin messenger RNA.

This is a short review on the isolation and translation in heterologous Systems of a 9 s RNA which carries the information of haemoglobin. Rabbit haemodobin (Hb) messenger RNA (mRNA) was the first eukaryotic genetic message to be isolated, quite a long time ago (1). This was mainly due to the fact that mammalian reticulocytes offer the favourable opportunity of being highly specialized cells wh...